General Cell Based Assays
Eurofins Discoverx cAMP Hunter eXpress CCR6 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0069E2CP2L)
The cAMP Hunter CCR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL20) based activation of CCR6 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CCR6, a naturally Gi/Go coupled GPCR. Upon activation of CCR6, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter IL7R/2RG Bioassay Kit, 10-Plate. (93-0997Y13-00082)
The PathHunter IL7R/IL2RG Dimerization Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-7) induced dimerization of IL7R/IL2RG receptors. This product uses EFC technology. The cell line included overexpresses IL7R target protein fused to PK alongside IL2RG target protein fused to EA. Upon ligand-induced dimerization of IL7R/IL2RG, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Cayman Chemical -NADP sodIum salt 3.8mg
A fluorescent derivative of NADP+; ex/em = 310/400 nm, respectively; has been used to determine the binding stoichiometry of NADP+ to FASN
Eurofins Discoverx PathHunter Human GHR-JAK1 Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human GHR-JAK1 Stable Cell Line Functional Kinase Assay (U2OS) measures GHR cytokine receptor activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human OPRM1 (Opioid mu) β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human OPRM1 (Opioid mu) β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure OPRM1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery U-PLEX Mouse IL-6 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-6 Assay Kit is a sensitive immunoassay for detecting mouse interleukin 6 (IL-6) in various samples It uses MSD s electrochemiluminescent technology offering high sensitivity a wide dynamic range and quick results The kit includes a 96-well plate with capture antibodies for mouse IL-6 detection antibodies with an electrochemiluminescent label and calibrators for a standard curve Sample incubation is 2 hours with no wash steps quantifying IL-6 from 0 2-10 000 pg/mL
Abcam Comet Assay Kit (3-well slides)
Comet Assay Kit (3-well slides)
The product is subject to the following: Abcam Restricted Use Statement
Miltenyi Biotec, Inc. IFN-g Secr Assay - Enr&Det Kit (PE), h
Isolation and detection of human IFN-γ–secreting cells.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
Eurofins Discoverx PathHunter eXpress IL23R/IL12RB1 Dimerization Assay, 10pl Kit. (93-1007E3CP0L)
The PathHunter IL23R/IL12RB1 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-23) induced dimerization of IL23R/IL12RB1 receptors. This product uses EFC technology. The cell line included overexpresses IL23R target protein fused to PK alongside IL12RB1 target protein fused to EA. Upon ligand-induced dimerization of IL23R/IL12RB1, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress AGTRL1 CHO-K1 β-Arrestin-1 GPCR Assay, 2pl Kit. (93-1050E2CP2M)
The PathHunter AGTRL1 (ARR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. apelin-13) based activation of AGTRL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AGTRL1 and EA-tagged β-Arrestin-1. Activation of AGTRL1 stimulates the recruitment of β-Arrestin-1 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam SREBP-2 Transcription Factor Assay Kit.
SREBP-2 Transcription Factor Assay Kit.
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical GlycolysIs asy Enzyme MIXu 1ea
A peptide agonist of MC1R induces cAMP accumulation in mouse L-cells expressing human MC1R (EC50 0.5 nM) induces tyrosinase activity in mouse melanoma cells induces long-term skin darkening in frogs at 1.8 mg/kg
Sigma Aldrich Fine Chemicals Biosciences SILuTMMAb K1 Pharmacokinet
All critical reagents necessary to perform the assay are provided in the kit. The plate-based format avoids the high cost and potential LC column blockage associated with bead-based enrichment formats. Sample preparation with the SILu(TM)MAb PK kit can be performed in under 5 hours and consists of coating wells with high-specificity capture antibody followed by immunoaffinity enrichment and rapid in-plate trypsin digestion. The resulting digest is ready for injection with no SPE or other cleanup required.
Eurofins Discoverx PathHunter eXpress PRLR-JAK1 Functional Assay, 10-Plate Kit. (93-0686E3CP0L)
The PathHunter PRLR-JAK1 Functional assay eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting CTKs. This kit assesses PRLR activation via detection of JAK1 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged PRLR cytokine receptor, an untagged CTK JAK1 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.